| Autor: |
S V, Bykovskaia, O V, Sysoev, Ia I, Bur'ianov |
| Rok vydání: |
1988 |
| Předmět: |
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| Zdroj: |
Prikladnaia biokhimiia i mikrobiologiia. 24(2) |
| ISSN: |
0555-1099 |
| Popis: |
A technique for quantification of S-adenosylmethionine in microbial cell-free extracts is proposed that involves dilution of S-adenosyl-L-(methyl-3H)methionine with non-labelled S-adenosylmethionine followed by DNA-cytosine-methyltransferase reaction. The content of S-adenosylmethionine and the activity of S-adenosylmethionine synthetase in yeasts and E. coli MRE-600 are in good agreement with the results obtained with labelled L-methionine and consistent with literature data. The sensitivity of the technique is about 0.1 nmol/0.1 ml of the reaction mixture (10(-6) M). The error was about 5% in every series of experiments. However, the combined use of different DNA-methyltransferase preparations resulted in a higher experimental error (up to 15%), which should be taken into consideration. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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