Autor: |
V P, Shevchenko, I Iu, Nagaev, P V, Vrzheshch, D E, Ershov, S V, Zaĭtsev |
Rok vydání: |
1988 |
Předmět: |
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Zdroj: |
Bioorganicheskaia khimiia. 14(8) |
ISSN: |
0132-3423 |
Popis: |
A method has been developed that makes it possible to obtain [5,6-3H2]PGE1 with a yield of 35% and a molar radioactivity of 1.7-1.8 TBq/mmol. The binding of [5,6-3H2]PGE1 to native platelets proved to be specific, saturating and reversible. It is characterized by low values (approximately 10(-9) M) of dissociation constants for high-affinity sites, correlates with the inhibition of ADP-induced aggregation of platelets and can be considered as receptor binding. Specific binding of 10 +/- 2 molecules of PGE1 with one platelet was found to cause 50% inhibition of the ADP-induced aggregation. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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