[Synthesis of tritium-labelled prostaglandin E1 and its binding to human platelets]

Autor: V P, Shevchenko, I Iu, Nagaev, P V, Vrzheshch, D E, Ershov, S V, Zaĭtsev
Rok vydání: 1988
Předmět:
Zdroj: Bioorganicheskaia khimiia. 14(8)
ISSN: 0132-3423
Popis: A method has been developed that makes it possible to obtain [5,6-3H2]PGE1 with a yield of 35% and a molar radioactivity of 1.7-1.8 TBq/mmol. The binding of [5,6-3H2]PGE1 to native platelets proved to be specific, saturating and reversible. It is characterized by low values (approximately 10(-9) M) of dissociation constants for high-affinity sites, correlates with the inhibition of ADP-induced aggregation of platelets and can be considered as receptor binding. Specific binding of 10 +/- 2 molecules of PGE1 with one platelet was found to cause 50% inhibition of the ADP-induced aggregation.
Databáze: OpenAIRE