[The effect of the amount of dialdehyde cellulose-bound enzyme on the activity of immobilized trypsin after gamma irradiation and in the storage process]

Autor:	A A, Belov, V V, Ryl'tsev, T E, Ignatiuk, V N, Filatov
Rok vydání:	1994
Předmět:	Drug Carriers Time Factors Gamma Rays Drug Storage Trypsin In Vitro Techniques Cellulose Crystallization Enzymes Immobilized Protein Binding
Zdroj:	Radiatsionnaia biologiia, radioecologiia. 34(2)
ISSN:	0869-8031
Popis:	It is found the complex effect of the bound enzyme concentration on the proteolytic activity of trypsin immobilized to dialdehydecellulose (preriodate oxidation) after gamma irradiation and in process of storage. It is shown the occurrence of three stages of immobilized enzyme inactivation in process of immobilization and storage. The velocity of inactivation did not depend on bound trypsin concentration. The ratio of proteolytic activity of samples before and after gamma irradiation was increased with the increase of immobilized to carrier enzyme concentration and was not change (in range of experiment error) in process of storage. The results were compared with that of crystalline trypsin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::be339363e8e808999536eaecdafaf911 https://pubmed.ncbi.nlm.nih.gov/8193725 Zobrazit plný text záznamu