| Autor: |
Maria Veronica, Lopez, Eduardo G, Cafferata, Diego L, Viale, Osvaldo L, Podhajcer |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1651 |
| ISSN: |
1940-6029 |
| Popis: |
Here we describe a collection of methods that have been adapted to isolate and modify tumor-specific promoters (TSPs ) to drive viral replication for cancer therapy and other uses. We will describe as examples the secreted protein acidic and rich in cysteine (SPARC ) and the protease-activated receptor-1 (PAR-1) promoter. We outline strategies to select appropriate TSPs using bioinformatics resources and the methods utilized in their subsequent cloning, assessment of transcriptional activity, and their use in conditionally replicative oncolytic adenoviruses . |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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