Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: a method for the removal of silver ions to enhance sensitivity

Autor:	F, Gharahdaghi, C R, Weinberg, D A, Meagher, B S, Imai, S M, Mische
Rok vydání:	1999
Předmět:	Ions Silver Staining Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Acrylic Resins Proteins Electrophoresis Gel Two-Dimensional Electrophoresis Polyacrylamide Gel Sensitivity and Specificity
Zdroj:	Electrophoresis. 20(3)
ISSN:	0173-0835
Popis:	Mass spectrometry is a powerful technique for the identification of proteins at nanogram quantities. However, some degree of sample preparation prior to mass spectrometry is required, and silver-stained protein gel samples are most problematic. Here we report our strategy to obtain peptide mass profiles from silver-stained protein gel samples from one- or two-dimensional gels by destaining prior to enzymatic digestion. This study demonstrates that by using the destaining method, the sensitivity and quality of mass spectra is increased for matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometric analysis, permitting more proteins to be identified by peptide mass database analysis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::be02e25836ed1cf3434cf5e6b3de1a19 https://pubmed.ncbi.nlm.nih.gov/10217175 Zobrazit plný text záznamu Plný text