IFN-gamma production and cytotoxicity of IL-2-activated murine NK cells are differentially regulated by MHC class I molecules
| Autor: | A, Kubota, R H, Lian, S, Lohwasser, M, Salcedo, F, Takei |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Cytotoxicity
Immunologic Binding Sites Membrane Glycoproteins Histocompatibility Antigens Class I Lymphocyte Activation Rats Killer Cells Natural Mice Inbred C57BL Interferon-gamma Mice Receptors KIR Tumor Cells Cultured Animals Antigens Ly Interleukin-2 Lectins C-Type Receptors Immunologic Receptors NK Cell Lectin-Like |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 163(12) |
| ISSN: | 0022-1767 |
| Popis: | Activation of NK cells by target cells leads to cytotoxicity as well as production of various cytokines including IFN-gamma. MHC class I molecules on target cells regulate NK cytotoxicity. However, little is known about the regulation of IFN-gamma production by NK cells. We examined the production of IFN-gamma in individual murine NK cells stimulated with tumor cell lines by flow cytometric analysis of intracellular IFN-gamma. Among several tumor lines tested, the rat basophilic leukemia line RBL-1 induced particularly high level of IFN-gamma production in IL-2-activated NK cells, whereas other lines, including the prototypic NK target YAC-1, induced very low or no IFN-gamma production. Transfection of murine classical MHC class I molecules into RBL-1 cells substantially inhibited IFN-gamma production. This inhibition of IFN-gamma production by MHC class I was independent of Ly-49 or CD94/NKG2A expression on NK cells. These results indicate that some target cells directly stimulate IL-2-activated NK cells and induce IFN-gamma production, but the requirements for the induction of IFN-gamma production seem different from those for NK cytotoxicity. Furthermore, similar to NK cytotoxicity, induction of IFN-gamma production is inhibited by MHC class I on stimulating cells. However, the MHC class I-specific receptors inhibiting IFN-gamma production are different from those for NK cytotoxicity. |
| Databáze: | OpenAIRE |
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