| Autor: |
Wen-yue, Yang, Lin, Cui, Ling-ling, Qu, Ning, Xu, Yun, Huang, Li-jian, Cui, Wen-hong, Zhan, Ding, Zhao |
| Rok vydání: |
2018 |
| Zdroj: |
Guang pu xue yu guang pu fen xi = Guang pu. 36(9) |
| ISSN: |
1000-0593 |
| Popis: |
The interactions of Salvianolic acis A (SAA) and Salvianolic acid B (SAB) with insulin were studied by using fluorescence spectroscopy, UV-vis spectroscopy and ATR-FTIR spectroscopy in simulating physiological condition (pH 7.40). The fluorescence quenching of insulin by SAA and SAB were static quenching process. The results of synchronous fluorescence and three-dimensional fluorescence spectra suggested no obvious conformation changes of insulin after SAA or SAB binding. But ATR-FTIR spectra showed that SAA and SAB could change the secondary structures of insulin, of which β-turns decreased and random coil increased accompanied with α-belices and β-sheets no clear change. The glucose might influenced the bioactivity of insulin in the SAA-insulin and SAB-insulin systems by changing the binding constants of SAA (or SAB) with insulin and exacerbating the changes of insulin conformation and relative contents of α-belices. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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