| Autor: |
Jun-Hui, Wang, Lei, Deng, Lu, Wang, Chen, Liang, Yi, Wang, Tie-Qiang, Liu, Shan, Huang, Ya-Jing, Huang, Bo, Cai, Zheng, Dong, Hong-Li, Zuo, Qi-Yun, Sun, Jian-Hui, Qiao, Chang-Lin, Yu, Kai-Xun, Hu, Hui-Sheng, Ai, Mei, Guo |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 25(3) |
| ISSN: |
1009-2137 |
| Popis: |
To explore the effect of infusing G-CSF mobilized recipient spleen cells at different time after haploidentical stem cell transplantation(HSCT) on graft-versus-host disease (GVHD) in mice and its possible mechanism.Forty mice after HSCT were randomly divided into 4 groups (n=10): GVHD positive control group (control group), 1st d recipient cell infusion group after transplantation (+1 d group), 4th d recipient cell infusion group after transplantation(+4 d group), 7th d recipient cell infusion group after transplantation(+7 d group). The mice in control group were injected the normal saline of same equivalent with experimental group which were given the same amount of G-CSF-mobilized recipient spleen cells. The general manifestation and pathological change of GVHD were observed. The expression changes of CD3The incidence of GVHD was significantly decreased in +4 d group and the median survival time was longer than 60 days, which was significantly higher than that of control group (24 d), +1 d group (21 d), +7 d group (28 d). (P0.01, P0.01, P0.01). The Fasl expression of peripheral blood T lymphocytes in +4 d group were significantly lower than that in the other 3 groups(P0.05).The +4 d infusion of G-CSF mobilized recipient spleen cells on 4th day after haploidentical HSC transplantation can inhibit the expression of FasL in donor T lymphocytes, and significantly reduce the incidence of GVHD. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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