Application of synthetic peptides to the diagnosis of neurocysticercosis

Autor:	Agnès, Fleury, Constantino, Beltran, Elizabeth, Ferrer, Terésa, Garate, Leslie J S, Harrison, R Michael E, Parkhouse, Esperanza, Garcia, Gladis, Fragoso, Julia, Costa-Cruz, Germano, Biondi, Svetlana, Agapejev, Edda, Sciutto
Rok vydání:	2003
Předmět:	Adult Male Molecular Sequence Data Antibodies Helminth Enzyme-Linked Immunosorbent Assay Taenia saginata DNA Helminth Middle Aged Neurocysticercosis Sensitivity and Specificity Peptide Fragments Antigens Helminth Taenia solium Humans Female Amino Acid Sequence Gene Library
Zdroj:	Tropical medicineinternational health : TMIH. 8(12)
ISSN:	1360-2276
Popis:	We tested the possible diagnostic utility of five Taenia saginata oncosphere-derived synthetic peptides in T. solium neurocysticercosis (NC). The five peptides correspond to protein sequences with high antigenic indexes that were cloned from a T. saginata oncosphere cDNA library. The test samples consisted of cerebrospinal fluid (CSF) samples randomly collected from patients referred from Mexican and Brazilian neurological institutes. Indirect enzyme-linked immunosorbent assays (ELISA) were carried out with the peptides either unconjugated or coupled to carrier proteins, and were compared with results obtained using T. solium cyst fluid as a positive control. For active inflammatory NC, the higher sensibility (93%) and specificity (85%) was obtained with peptides HP6-2 and Ts45W-1, respectively, coupled to ovalbumin, in both Mexican and Brazilian patients. Examining the results of the individual peptide assays in combination, in some instances, improved the sensitivity to 100%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::bd3d3bf94c150439eb9c93e41471d246 https://pubmed.ncbi.nlm.nih.gov/14641848 Zobrazit plný text záznamu Plný text