Inhibition of agonist-induced activation of phospholipase C following poxvirus infection
| Autor: | K G, Oliver, R M, Buller, P J, Hughes, J W, Putney, G J, Palumbo |
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| Rok vydání: | 1992 |
| Předmět: |
Amanitins
Inositol Phosphates Vaccinia virus Inositol 1 4 5-Trisphosphate Cell Transformation Viral Kidney Cell Line Enzyme Activation Kinetics Adenosine Triphosphate Type C Phospholipases Chlorocebus aethiops Carcinoma Squamous Cell Tumor Cells Cultured Animals Humans Calcium RNA Messenger Cycloheximide Inositol |
| Zdroj: | The Journal of biological chemistry. 267(35) |
| ISSN: | 0021-9258 |
| Popis: | Recent studies indicate that viruses may influence polyphosphoinositide levels. This study has examined the effects of vaccinia virus infection on phospholipase C activity. Infection of BS-C-1 cells, an African Green Monkey kidney cell line, or A431 cells, a human carcinoma cell line, with vaccinia virus inhibits receptor-mediated phospholipase C activation. As a consequence, agonist-mediated Ca2+ mobilization in BS-C-1 cells also was inhibited by vaccinia virus infection. Alleviation of the inhibition of phospholipase C activation was observed in vaccinia virus-infected cells treated with cycloheximide without influencing uninfected cells. Treatment of infected cells with alpha-amanitin, an inhibitor of host mRNA synthesis but not virus mRNA synthesis, failed to alleviate the inhibition of phospholipase C activation. Together these results suggest that a virus-encoded gene product mediates the inhibition of phospholipase C activation without the need of a virus-induced host factor. Analysis of the processes involved in the formation of inositol (1,4,5)-trisphosphate and mobilization of intracellular Ca2+ indicate that the vaccinia virus gene product exerts its inhibitory effects at the level of phospholipase C activity. This may occur by either directly reducing the amount of phospholipase C, reducing the specific activity of phospholipase C, or by inhibiting the association of phospholipase C with its substrate, phosphatidylinositol 4,5-bisphosphate. |
| Databáze: | OpenAIRE |
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