Transcriptome Analysis of Testis from HFD-Induced Obese Rats (
| Autor: | Ahmed M, El-Shehawi, Samir, El-Shazly, Mohamed, Ahmed, Mohamed, Alkafafy, Samy, Sayed, Samy, Farouk, Saqer S, Alotaibi, Mona M, Elseehy |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
obesity Gene Expression Diet High-Fat Article transcriptome analysis Testis Animals Genetic Predisposition to Disease Testosterone Obesity Rats Wistar Infertility Male Sertoli Cells Gene Expression Profiling High-Throughput Nucleotide Sequencing Spermatozoa spermatogenesis Rats Gene Ontology Gene Expression Regulation gene expression RNA Transcriptome infertility Biomarkers |
| Zdroj: | International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Popis: | Obesity is a worldwide life-threatening metabolic disorder, associated with various chronic diseases, including male infertility. Obesity was induced by high fat diet (HFD), and testis RNA was used for the transcriptome analysis using RNAseq via Illumina NovaSeq 6000 System and NovaSeq 6000 Kit. Gene expression level was estimated as FPKM (Fragments Per Kilobase of transcript per Million mapped reads). Differential expressed genes (DEGs) were annotated against gene ontology (GO) and KEGG databases. More than 63.66 million reads per sample were performed with 100 bp cutoff and 6 Gb sequencing depth. Results of this study revealed that 267 GO terms (245 biological processes (BP), 14 cellular components (CC), eight molecular functions (MF)), and 89 KEGG pathways were significantly enriched. Moreover, total numbers of 136 genes were differentially expressed (107 upregulated, 29 downregulated) with |FC| ≥ 2 and bh adjusted |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |