Comparative genomic hybridization of human malignant gliomas reveals multiple amplification sites and nonrandom chromosomal gains and losses
| Autor: | E, Schröck, G, Thiel, T, Lozanova, S, du Manoir, M C, Meffert, A, Jauch, M R, Speicher, P, Nürnberg, S, Vogel, W, Jänisch |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Adolescent Chromosome Disorders Astrocytoma Image Processing Computer-Assisted Tumor Cells Cultured Humans Child neoplasms Interphase In Situ Hybridization Fluorescence Aged Chromosome Aberrations Chromosomes Human Pair 10 Genome Human Gene Amplification Chromosome Mapping DNA Neoplasm Glioma Middle Aged DNA Fingerprinting Chromosome Banding Microscopy Electron Female Glioblastoma Chromosomes Human Pair 7 Regular Articles |
| Zdroj: | The American journal of pathology. 144(6) |
| ISSN: | 0002-9440 |
| Popis: | Nine human malignant gliomas (2 astrocytomas grade III and 7 glioblastomas) were analyzed using comparative genomic hybridization (CGH). In addition to the amplification of the EGFR gene at 7p12 in 4 of 9 cases, six new amplification sites were mapped to 1q32, 4q12, 7q21.1, 7q21.2-3, 12p, and 22q12. Nonrandom chromosomal gains and losses were identified with overrepresentation of chromosome 7 and underrepresentation of chromosome 10 as the most frequent events (1 of 2 astrocytomas, 7 of 7 glioblastomas). Gain of a part or the whole chromosome 19 and losses of chromosome bands 9pter-23 and 22q13 were detected each in five cases. Loss of chromosome band 17p13 and gain of chromosome 20 were revealed each in three cases. The validity of the CGH data was confirmed using interphase cytogenetics with YAC clones, chromosome painting in tumor metaphase spreads, and DNA fingerprinting. A comparison of CGH data with the results of chromosome banding analyses indicates that metaphase spreads accessible in primary tumor cell cultures may not represent the clones predominant in the tumor tissue. |
| Databáze: | OpenAIRE |
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