| Autor: |
Jun, Ma, Wen, Qu, Jing-Lian, Tao, Wei-Wei, Qi, Hua-Quan, Wan, Li-Min, Xing, Jin, Chen, Xi-Feng, Dong, Yang, Zhang, Zhao-Yun, Liu, Yi-Hao, Wang, Hui, Liu, Hong, Liu, Li-Juan, Li, Rong, Fu, Zong-Hong, Shao |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 28(5) |
| ISSN: |
1009-2137 |
| Popis: |
To investigate the expression of IL-9 and IL-6 in patients with BCR-ABL- bone marrow proli- ferative tumor (MPN), and to explore its role in the occurrence and development of MPN.A total of 71 newly diagnosis MPN patients treated in Tianjin Medical University General Hospital from 2018 to 2019 were selected, including 32 patients with polycythemia vera (PV) and 22 patients with primary thrombocytosis (ET), and 17 patients with primary myelofibrosis (PMF). Then 58 patients who retestine after treatment were selected as therapy group，and 20 healthy volunteers were recruited as control group. ELISA was used to detect the expression level of IL-6 and IL-9 in bone marrow supernatant, and the relative expression level of IL-6 and IL-9 mRNA in BMMNC was detected by real-time PCR. The proportion of Th9 cells in peripheral blood were detected by flow cytometry (FCM). The expression level of IL-6 mRNA and IL-9 mRNA of BMMNC and clinical indicators were analyzed, and the correlation between JAK2 gene mutation load and IL-9 level was further analyzed.The level of IL-6 in bone marrow supernatant and the expression of IL-6 mRNA in BMMNC were higher in the newly diagnosed group as compared with those in the treated group and the control group (P0.001). The expression level of IL-9 in bone marrow supernatant and the expression of IL-9 mRNA in BMMNC were lower in the newly diagnosed group as compared with those in the treated group and the control group (P0.05). The proportion of Th9 cells in peripheral blood was lower in the newly diagnosed group as compared with that in the treated group and the control group (P0.001). The level of IL-6 in bone marrow supernatant and the expression of IL-6 mRNA in BMMNC in JAK2The increased expression of IL-6 in MPN and hyperfunction may promote the progression of BCR-ABL- MPN disease. The expression of IL-9 in MPN decreases, and it negatively correlates with the mutation load of JAK2 gene, which may be related with the decrease of tumor environmental antitumor immune effect.IL-9和IL-6在BCR-ABL- MPN患者中的表达及其意义.检测BCR-ABL- 骨髓增殖性肿瘤（MPN）患者的IL-9和IL-6的表达，探讨其在MPN疾病中的意义.选取2018年至2019年间于天津医科大学总医院血液科就诊的MPN初治患者71例（初诊组），包括32例真性红细胞增多症（PV），22例原发性血小板增多症（ET）和17例原发性骨髓纤维化（PMF），治疗后复查患者58例作为治疗组，同时选取健康志愿者20名作为对照组。采用ELISA法检测骨髓上清液中IL-6和IL-9的水平，采用RT-PCR技术检测骨髓单个核细胞IL-6和IL-9 mRNA相对表达水平，应用流式细胞术(FCM)检测外周血Th9细胞的比例，将骨髓单个核细胞IL-6 mRNA和IL-9 mRNA表达水平与临床指标进行相关性分析，并进一步追踪分析JAK2基因突变负荷与IL-9水平之间的关系.初诊组骨髓上清液中IL-6水平及骨髓单个核细胞中IL-6 mRNA表达水平均高于治疗组和对照组（P0.001），初诊组骨髓上清液中IL-9水平及骨髓单个核细胞中IL-9 mRNA表达水平均低于治疗组和对照组（P0.05）；初诊组外周血Th9细胞比例低于治疗组和对照组（P0.001）；JAK2IL-6在MPN中表达水平增加，功能亢进，可能促进BCR-ABL- MPN疾病进展；IL-9在MPN中表达水平减低，且与JAK2基因突变负荷呈负相关关系，这可能与肿瘤微环境抗肿瘤免疫作用减低有关. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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