[Humoral response to the injection of acellular Pertussis vaccine]
| Autor: | N S, Zakharova, T N, Remova, M V, Britsina, E I, Shmeleva, N U, Mertsalova, E M, Zaĭtsev, M N, Ozeretskovskaia, I G, Bazhanova, E V, Ermolova |
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| Rok vydání: | 2002 |
| Předmět: |
Pertussis Vaccine
Whooping Cough Vaccination CHO Cells Hemagglutination Tests Antibodies Bacterial Bordetella pertussis Immunoenzyme Techniques Mice Hemagglutinins Vaccines Acellular Pertussis Toxin Vaccines Inactivated Neutralization Tests Cricetinae Animals Immunization Rabbits Virulence Factors Bordetella Adhesins Bacterial |
| Zdroj: | Zhurnal mikrobiologii, epidemiologii i immunobiologii. (6) |
| ISSN: | 0372-9311 |
| Popis: | The humoral response of mice and rabbits to the injection of whole-cell pertussis vaccine (PV) and acellular pertussis vaccine (APV), developed at the Mechnikov Research Institute for Vaccines and Sera (Russian Acad. Med. Sci.) in Moscow, was studied. In the sera of immunized animals antibodies to the antigenic complex were determined in the direct hemagglutination (DHA) test, specific antibodies to filamentous hemagglutinin (FHA) and pertussis toxin (PT)--in the enzyme immunoassay (EIA) and antibodies neutralizing PT in a cytopathogenic dose (CPD)--in neutralization test on Chinese hamster ovary (CHO) cells. In mice and rabbits immunized with APV the antibody titers determined in the DHA test were higher than those in the animals immunized with PV. Specific antibodies titers to FHA and PT in the sera of rabbits immunized with APV were also higher than those in the sera of rabbits immunized with PV. High dilutions of sera taken from the animals immunized with APC neutralized 4-16 doses of PT in the neutralization test on CHO cells. The most important result of this study was the detection of a more pronounced immune response in the animals immunized with APV in comparison with that induced by PV according to the results obtained in EIA and in the test of PT CPD neutralization on CHO cells. |
| Databáze: | OpenAIRE |
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