| Autor: |
G I, Lepesheva, I V, Turko, I A, Ges', V L, Chashchin |
| Rok vydání: |
1994 |
| Předmět: |
|
| Zdroj: |
Biokhimiia (Moscow, Russia). 59(7) |
| ISSN: |
0320-9725 |
| Popis: |
The possibilities of direct antigen detection in unlabelled systems based on immunoglobulin G Langmuir-Blodgett films as a sensitive surface have been studied. It was shown that an increase in the monolayer number in an immunoglobulin G Langmuir-Blodgett film deposited onto a solid surface coated with a thin silver film (50 nm) resulted in the regeneration of the antigen-binding capacity of the upper antibody layer. This dependence can be used for the construction of a direct optical immunosensor based on surface plasmon resonance. Moreover, a model of a piesoelectric immunosensor on the basis of immunoglobulin G Langmuir-Blodgett films for direct ferritin detection has been proposed. The detection range of ferritin concentrations in solution is 10(-10)-10(-7) M. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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