| Autor: |
T A, Shamolina, V K, Svedas |
| Rok vydání: |
2000 |
| Předmět: |
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| Zdroj: |
Biochemistry. Biokhimiia. 65(6) |
| ISSN: |
0006-2979 |
| Popis: |
Individual subunits of penicillin acylase from E. coli were isolated by gel-filtration under denaturing conditions (8 M urea). Recovery of the catalytic activity of the penicillin acylase heterodimer was studied after removal of urea. In the case of the heterodimer, 40-60% of the initial activity was recovered, whereas the activity of individual subunits was not recovered. Combination of native enzyme subunits with subunits isolated from the enzyme pre-inactivated with the irreversible inhibitor phenylmethylsulfonyl fluoride resulted in heterodimers which were active only in the case of involvement of the beta-subunit of the active enzyme. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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