| Autor: |
J-X, Zhang, J-F, Zhai, X-T, Yang, J, Wang |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
European review for medical and pharmacological sciences. 20(18) |
| ISSN: |
2284-0729 |
| Popis: |
Increasing evidence shows that microRNA involves in the development of several types of cancers, however, the role of microRNA-132 (miR-132) in non-small cell lung cancer (NSCLC) metastasis remains largely unknown. In this study, we aimed to investigate the effect of miR-132 on the epithelial-mesenchymal transition (EMT) and the potential mechanisms in NSCLC.The Quantitative real-time PCR (QRT-PCR) was used to detect the miR-132 levels in 15 NSCLC tissues and cell lines. Transwell and wound healing assays were used to evaluate the function of miR-132 in NSCLC cell metastasis. EMT-related markers were determined by using qRT-PCR. EMT-related TGFβ1/Smad2 signaling pathway was explored using Western blot.MiR-132 expression level was lower in NSCLC tissues compared with the matched adjacent normal tissues. It was also downregulated in A549 cell lines compared to normal lung epithelial cell BEAS-2B. MiR-132 overexpression obviously inhibited migration and invasion capacities in A549 cells while miR-132 down-regulation would enhance such capacities. Expression of EMT-related markers and TGFβ1/Smad2 was higher in A549 cells transfected with miR-132 inhibitor compared with those transfected with miR-132 mimic. Moreover, expression of EMT-related markers and Smad2 was increased in NSCLC tissues compared to in the adjacent normal tissues and the reverse expression of miR-132 and Smad2 was observed.These results indicate that miR-132 may play a suppressive role in the metastasis of NSCLC cells by promoting EMT via TGFβ1/Smad2 signaling pathway. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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