L-cysteine increases glucose uptake in mouse soleus muscle and SH-SY5Y cells
| Autor: | Vered, Gazit, Ron, Ben-Abraham, Oded, Vofsi, Yeshayahu, Katz |
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| Rok vydání: | 2003 |
| Předmět: |
Mice
Inbred ICR Glucose Transporter Type 4 Glucose Transporter Type 3 Monosaccharide Transport Proteins Blotting Western Muscle Proteins Nerve Tissue Proteins Kinetics Mice Neuroblastoma Glucose Xanthenes Oxazines Tumor Cells Cultured Animals Humans Cysteine Coloring Agents Muscle Skeletal Cells Cultured |
| Zdroj: | Metabolic brain disease. 18(3) |
| ISSN: | 0885-7490 |
| Popis: | Previous investigation demonstrated the potential of L-cysteine (L-Cys) at high concentrations to cause hypoglycemia in mice totally deprived of insulin. For further elucidation of the glucose-lowering mechanism, glucose uptake and quantity of glucose transporters (GLUTs 3 and 4) in mouse soleus muscle and C2C12 muscle cells, as well as in human SH-SY5Y neuroblastoma cells, were investigated. A marked enhancement of glucose uptake was demonstrated, peaking at 5.0 mM L-Cys in soleus muscle (P0.05) and SH-SY5Y cells (P0.001), respectively. In contrast, glucose uptake was not affected in the C2C12 muscle cells. Kinetic analysis of the SH-SY5Y glucose uptake showed a 2.5-fold increase in maximum transport velocity compared with controls (P0.001). In addition, both GLUT3 and GLUT4 levels were increased following exposure to L-Cys. Our findings point to a possible hypoglycemic effect of L-Cys. |
| Databáze: | OpenAIRE |
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