[Human B-lymphoblastoid cell lines: optimal conditions for their procurement, selection, cultivation and cloning]

Autor: E I, Deriugina, N I, Olovnikova, L N, Lemeneva, N I, Drize, E V, Belkina, I L, Chertkov
Rok vydání: 1991
Předmět:
Zdroj: Gematologiia i transfuziologiia. 36(8)
ISSN: 0234-5730
Popis: The protocol of Epstein-Barr virus (EBV)-induced transformation of B-lymphocytes from reripheral blood of normal humans has been described. The requirements for mononuclear EBV-infection and for selection, cultivation, and cloning of EBV-transformed cell lines have been investigated. Successful establishment of stable human immunoglobulin-producing cell lines from lymphocytes of immunized donors could be achieved following the protocol presented.
Databáze: OpenAIRE