Separation and Characterization of Protein-DNA Complexes by EMSA and In-Gel Footprinting
Autor: | Daniel, Charlier, Indra, Bervoets |
---|---|
Rok vydání: | 2022 |
Předmět: | |
Zdroj: | Methods in molecular biology (Clifton, N.J.). 2516 |
ISSN: | 1940-6029 |
Popis: | In-gel footprinting enables the precise identification of protein binding sites on the DNA after separation of free and protein-bound DNA molecules by gel electrophoresis in native conditions and subsequent digestion by the nuclease activity of the 1,10-phenanthroline-copper ion [(OP) |
Databáze: | OpenAIRE |
Externí odkaz: |