Separation and Characterization of Protein-DNA Complexes by EMSA and In-Gel Footprinting

Autor: Daniel, Charlier, Indra, Bervoets
Rok vydání: 2022
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 2516
ISSN: 1940-6029
Popis: In-gel footprinting enables the precise identification of protein binding sites on the DNA after separation of free and protein-bound DNA molecules by gel electrophoresis in native conditions and subsequent digestion by the nuclease activity of the 1,10-phenanthroline-copper ion [(OP)
Databáze: OpenAIRE