Transcription factor RUNX3 promotes CD8
| Autor: | Qian, Song, Jun, Shang, Chufan, Zhang, Jianing, Chen, Lanlin, Zhang, Xianghua, Wu |
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| Rok vydání: | 2019 |
| Předmět: |
Chemokine CCL20
Lung Neoplasms Transcription Genetic Chemotaxis Adenocarcinoma of Lung CD8-Positive T-Lymphocytes Immunohistochemistry Up-Regulation Core Binding Factor Alpha 3 Subunit A549 Cells Cell Movement Cell Line Tumor Immune System Tumor Microenvironment Humans RNA-Seq Chemokines Chemokine CCL3 |
| Zdroj: | Journal of cellular biochemistryREFERENCES. 121(5-6) |
| ISSN: | 1097-4644 |
| Popis: | Considering the existence of immune-desert in tumor microenvironment, the clinical efficacy of immunotherapy for lung adenocarcinoma is limited. This study aims to investigate the ability of transcription factors in regulating tumor immune microenvironment in lung adenocarcinoma. RNA-seq data were collected from the The Cancer Genome Atlas database. The relationships between transcription factors and immune infiltrates were assessed. Runt-related transcription factor 3 (RUNX3)-associated immune pathways were investigated by the Kyoto Encyclopedia of Genes and Genomes, Gene Ontology, and Gene set enrichment analysis. Upregulated chemokines in the RUNX3-overexpressed cell line were determined by quantitative real-time polymerase chain reaction, western blot, and enzyme-linked immunosorbent assay. These chemokines were further confirmed in RUNX3-downregulated cell lines. Immunochemistry was conducted to determine the expression of RUNX3, CCL3, CCL20, and the numbers of CD8 |
| Databáze: | OpenAIRE |
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