| Autor: |
Heng, Zhong, Sivamani, Elumalai, Samson, Nalapalli, Lee, Richbourg, Anna, Prairie, David, Bradley, Shujie, Dong, Xiujuan Jenny, Su, Weining, Gu, Tim, Strebe, Liang, Shi, Qiudeng, Que |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1676 |
| ISSN: |
1940-6029 |
| Popis: |
One of the major limitations of maize transformation is the isolation of a large number of immature embryos using the time-consuming manual extraction method. In this article, we describe a novel bulk embryo extraction method for fast isolation of a large number of embryos suitable for both biolistic- and Agrobacterium-mediated transformation. Optimal gene delivery and tissue culture conditions are also described for achieving high efficiency in Agrobacterium-mediated maize transformation using phosphomannose isomerase (PMI) as a selectable marker. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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