| Autor: |
Yurii V, Stepanov, Iuliia, Golovynska, Sergii, Golovynskyi, Liudmyla V, Garmanchuk, Oleksandr, Gorbach, Liudmyla I, Stepanova, Natalia, Khranovska, Liudmyla I, Ostapchenko, Tymish Y, Ohulchanskyy, Junle, Qu |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Journal of photochemistry and photobiology. B, Biology. 227 |
| ISSN: |
1873-2682 |
| Popis: |
Irradiation with red or near-infrared (NIR) light in low level light therapy (LLLT) is found to stimulate cellular processes and bioenergetics, resulting in enhanced wound healing, pain control, neurodegenerative diseases treatment, etc. During light irradiation of tissues and organs, different cells are affected, though the connection between photostimulation of cells and their environmental conditions remains poorly understood. In this report, red/NIR light-stimulated angiogenesis is investigated using endothelial cells in vitro, with a focus on the capillary-like structure (CLS) formation and the respective biochemical processes in cells under conditions proximate to a healthy or malignant environment, which strongly defines angiogenesis. To model environmental conditions for endotheliocytes in vitro, the cell culture environment was supplemented by an augmented conditioned medium from macrophages or cancer cells. The biochemical processes in endothelial cell cultures were investigated with and without irradiation by red (650 nm) and near-infrared (808 nm) laser diodes and under normoxia or hypoxia conditions. A light-stimulated angiogenesis has been found, with a more efficient stimulation by 650 nm light compared to 808 nm light. It was shown that the irradiation with light promoted extracellular Ca |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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