High P-glycoprotein-mediated export observed in patients with a history of idiopathic thrombocytopenic purpura
| Autor: | Adam S, Levy, Susanna, Cunningham-Rundles, BethAnne, Mazza, Maciej, Simm, Richard, Gorlick, James, Bussel |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Purpura Thrombocytopenic Idiopathic Adolescent Reverse Transcriptase Polymerase Chain Reaction Cell Culture Techniques Syndrome Middle Aged Thrombocytopenia Autoimmune Diseases Humans Female Rhodamine 123 ATP Binding Cassette Transporter Subfamily B Member 1 Lymphocytes Aged Fluorescent Dyes |
| Zdroj: | British journal of haematology. 118(3) |
| ISSN: | 0007-1048 |
| Popis: | Studies have suggested that high P-glycoprotein expression in lymphocytes from patients with autoimmune disorders may affect disease outcome. Idiopathic thrombocytopenic purpura (ITP) and Evans' syndrome are widely thought to be autoimmune processes, however, the precise mechanisms remain unknown. Peripheral blood mononuclear cells from patients with refractory or recurrent ITP or Evans' syndrome were studied using the rhodamine 123 flow cytometric assay to investigate functional export levels. Lymphocytes from ITP and Evans' syndrome patients showed a significantly decreased ability to retain rhodamine, suggesting increased export protein function. Reverse transcription polymerase chain reaction distinguished P-glycoprotein as the likely export protein. |
| Databáze: | OpenAIRE |
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