| Autor: |
Prause, Stefan, Eidens, Moritz, Weise, Alexander, Matthias, Weber, Dahmen, Norbert, Wunsch, Annette, Forst, Thomas, Pfützner, Andreas |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Clinical laboratory. 55(9-10) |
| ISSN: |
1433-6510 |
| Popis: |
The human dopamine receptor D2 (DRD2) is known to be involved in several mental disorders. A series of studies revealed that the polymorphisms *TaqIA and *C957T are linked to these disorders and might influence drug response.Screening methods were established for the *TaqIA and *C957T polymorphisms. In the past, the technique of choice has been the time and resource consuming PCR with restriction fragment length polymorphism (PCR-RFLP). It served as the reference method for this investigation.A protocol was successfully developed for detecting *C957T and *TaqIA on the Roche LightCycler system. In addition, an allele frequency study was conducted comparing 98 samples from healthy and 86 samples from mentally disordered subjects for the *C957T and *TaqIA polymorphisms. Allele frequencies in the healthy population turned out to be 50% for the *C957T and 18% for the *TaqIA polymorphism. In the mentally disordered group, allele frequencies occurred with 54% for the *C957T and with 21% for the *TaqIA polymorphism.The protocol developed for identifying the *C957T and *TaqIA polymorphisms is suitable for high-throughput screening. Findings of the allele frequency study did not reveal any significant difference in the prevalence of the investigated polymorphisms. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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