| Autor: |
Li-Li, Wang, Chang-Yun, Ye, Yan-Mei, Xu, Zhi-Gang, Cui, Huai-Qi, Jing, Dong, Jin, Hua-Mao, Du, Shou-Yin, Zhang, Xue-Mei, Bai, Ai-Lan, Zhao, Jian-Guo, Xu |
| Rok vydání: |
2008 |
| Předmět: |
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| Zdroj: |
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi. 29(5) |
| ISSN: |
0254-6450 |
| Popis: |
To develop a PFGE protocol for Streptococcus suis.We developed and optimized a PFGE protocol for S. suis, in terms of plug preparation, choice of restriction endonucleases and optimized electrophoresis parameters. By analyzing the genome sequences of S. suis P1/7 with Mapdraw of DNAStar, we found three restriction enzymes, Swa I, Sma I and Apa I, were more suitable than others.Analysis of 100 isolates of S. suis including 34 of 35 serotypes identified, 59, 53 and 43 patterns were obtained from Swa I, Sma I and Apa I restriction, respectively. The enzyme Swa I had the greatest power for discrimination ability.By optimization of the protocol at various conditions, a rapid, reproducible, economic and practical PFGE method for S. suis was developed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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