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To study the characteristics of soil nutrients and fungal community composition under different rotation patterns in the arid zone of central Ningxia, we used millet rotation soybean (MRG), rotation grain amaranth (MRA), rotation quinoa (MRQ), and continuous millet rotation (CK) as the objects. The soil nutrient content was determined, and the sequence of the ITS variation region of soil fungi was determined using the Illumina MiSeq high-throughput sequencing platform. The results showed that the effects of different rotation patterns on the soil nutrients were different. The soil pH and electrical conductivity decreased under three rotation patterns, and the soil total nitrogen, total potassium, total phosphorus, and organic matter contents increased. The number of OTUs and |