Intestinal epithelial ablation of Pit-2/Slc20a2 in mice leads to sustained elevation of vitamin D

Autor:	Eva M, Pastor-Arroyo, Thomas, Knöpfel, Pedro H, Imenez Silva, Udo, Schnitzbauer, Nadège, Poncet, Jürg, Biber, Carsten A, Wagner, Nati, Hernando
Rok vydání:	2020
Předmět:	Intestines Mice Sodium-Phosphate Cotransporter Proteins Type III Animals Sodium-Phosphate Cotransporter Proteins Type IIb Cholecalciferol Diet Phosphates
Zdroj:	Acta physiologica (Oxford, England)REFERENCES. 230(2)
ISSN:	1748-1716
Popis:	Several NaIntestinal epithelial Pit-2-deficient mice were generated by crossing floxed Pit-2 with villin-Cre mice. Mice were fed 2 weeks standard or low phosphate diets. Stool, urine, plasma and intestinal and renal tissue were collected. Concentration of electrolytes and hormones, expression of mRNAs and proteins and intestinal transport of tracers were analysed.Intestinal mRNA expression of NaPi-IIb and Pit-1 is segment-specific, whereas the abundance of Pit-2 mRNA is more homogeneous. In ileum, NaPi-IIb mRNA expression is restricted to enterocytes, whereas Pit-2 mRNA is found in epithelial and non-epithelial cells. Overall, their mRNA expression is not regulated by dietary phosphate. The absence of Pit-2 from intestinal epithelial cells does not affect systemic phosphate homeostasis under normal dietary conditions. However, in response to dietary phosphate restriction, Pit-2-deficient mice showed exacerbated hypercalciuria and sustained elevation of 1,25(OH)In mice, the intestinal Na
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::ba21922fbbedabe205a6cfb6a4377780 https://pubmed.ncbi.nlm.nih.gov/32564464 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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