| Autor: |
Pierre, Luciano, Stéphane, Coulon, Virginie, Faure, Yves, Corda, Julia, Bos, Steven J, Brill, Eric, Gilson, Marie-Noelle, Simon, Vincent, Géli |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
The EMBO journal. 31(8) |
| ISSN: |
1460-2075 |
| Popis: |
In Saccharomyces cerevisiae, the telomerase complex binds to chromosome ends and is activated in late S-phase through a process coupled to the progression of the replication fork. Here, we show that the single-stranded DNA-binding protein RPA (replication protein A) binds to the two daughter telomeres during telomere replication but only its binding to the leading-strand telomere depends on the Mre11/Rad50/Xrs2 (MRX) complex. We further demonstrate that RPA specifically co-precipitates with yKu, Cdc13 and telomerase. The interaction of RPA with telomerase appears to be mediated by both yKu and the telomerase subunit Est1. Moreover, a mutation in Rfa1 that affects both the interaction with yKu and telomerase reduces the dramatic increase in telomere length of a rif1Δ, rif2Δ double mutant. Finally, we show that the RPA/telomerase association and function are conserved in Schizosaccharomyces pombe. Our results indicate that in both yeasts, RPA directly facilitates telomerase activity at chromosome ends. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Plný text