Preparation and in vitro anti-tumor properties of toad venom extract-loaded solid lipid nanoparticles
| Autor: | Su-Juan, Zhang, Yong-Tai, Zhang, Ji-Hui, Zhao, Li-Na, Shen, Feng, Shi, Nian-Ping, Feng |
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| Rok vydání: | 2013 |
| Předmět: |
Ovarian Neoplasms
Chemistry Pharmaceutical Drug Compounding Cell Cycle Tetrazolium Salts Uterine Cervical Neoplasms Antineoplastic Agents Administration Intravaginal Thiazoles Drug Delivery Systems Microscopy Electron Transmission Suspensions Drug Design Amphibian Venoms Humans Nanoparticles Female Particle Size Chromatography High Pressure Liquid HeLa Cells |
| Zdroj: | Die Pharmazie. 68(8) |
| ISSN: | 0031-7144 |
| Popis: | In this study, we prepared solid lipid nanoparticles (TV-SLNs) loaded with toad venom extract and investigated their anti-tumor effects in vitro in HeLa and SKOV-3 cells. TV-SLNs were prepared using a cold homogenization technique, and the formulation was optimized by central composite design and response surface methods. The anti-tumor activities of TV-SLNs were evaluated by analyzing cell division and cell cycle distribution by using the MTT assay and flow cytometry. After incubation with TV-SLNs, the growth of both HeLa and SKOV-3 cells was inhibited significantly. The percentage of HeLa cells in G0/G1 phase decreased, whereas that in the S and G2/M phases increased. Thus, the S and G2/M phases were blocked after the incubation of HeLa cells with TV-SLNs for 24 h. In contrast, the percentage of SKOV-3 cells in G0/G1 phase increased and then decreased in S and G2/M phases, with the G0/G1 phase being blocked after incubation with TV-SLNs for 24 h. Our results demonstrate that TV-SLNs inhibited the fissiparism of HeLa and SKOV-3 cells in a time-and dose-dependent manner. TV-SLNs may be effective as a novel TV vaginal delivery system for the treatment of cervical and ovarian cancers. |
| Databáze: | OpenAIRE |
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