Preparative separation of intact incomplete and empty adenovirus type 2 particles

Autor: M, Tóth, B, Taródi, I, Béládi
Rok vydání: 1982
Předmět:
Zdroj: Acta virologica. 26(4)
ISSN: 0001-723X
Popis: Complete adenovirus type 2 virions containing the whole genome were resistant to while empty capsids lacking DNA were breakable at a separation procedure involving customary CsCl gradient centrifugation. A purification method was developed using CsCl density gradient centrifugation in 5% glycerol which retained the integrity of incomplete particles as well as empty capsids. This procedure enabled to study the biological and physicochemical properties of different adenovirus type 2 particles.
Databáze: OpenAIRE