| Autor: |
S, Sievers, M, Cretich, P, Gagni, B, Ahrens, G, Grishina, H A, Sampson, B, Niggemann, M, Chiari, K, Beyer |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. 47(8) |
| ISSN: |
1365-2222 |
| Popis: |
Microarray-based component-resolved diagnostics (CRD) has become an accepted tool to detect allergen-specific IgE sensitization towards hundreds of allergens in parallel from one drop of serum. Nevertheless, specificity and sensitivity as well as a simultaneous detection of allergen-specific IgGWe applied the recently introduced silicon chip coated with a functional polymer named copoly(DMA-NAS-MAPS) to the simultaneous detection of food allergen-specific IgE and IgGNative and recombinant allergenic proteins from hen's egg and cow's milk were spotted on silicon chips coated with copoly(DMA-NAS-MAPS) along with known concentrations for human IgE and IgGAllergen-specific IgE and IgGWe confirmed the ability of the polymer coated silicon microarray to be comparably sensitive to the ImmunoCAP ISAC for various food allergens. This suggests that the copoly(DMA-NAS-MAPS) microarray is a low-cost, self-producible alternative to the commercial ImmunoCAP ISAC in allergy research. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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