| Autor: |
M A, Vladimirskiĭ, L I, Mordovskaia, V A, Aksenova, L K, Shipina, E I, Aksenova, O V, Sergienko, A M, Liashuk, T V, Neretina, A S, Kariagina, V G, Lunin, E E, Efremov, G V, Ignashenkova, T N, Vlasik, E V, Ianushevskaia, N M, Kashirina |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Klinicheskaia laboratornaia diagnostika. (1) |
| ISSN: |
0869-2084 |
| Popis: |
A test system was developed to detect tuberculous infection by qualitative analysis of interferon-gamma (IFN-gamma) in the plasma samples after 20-24-hour incubation of whole blood samples in the presence of Mycobacterium tuberculosis (MBT) antigens: tuberculin PPD and a mixture of the MBT-specific recombinant antigens ESAT-6 and CFP-10. The analysis used 3 test tubes each containing 1 ml of heparinized venous blood, one of which served as a control; the other two test tubes were employed to measure antigen-induced IFN-gamma production. Whether this test system might be used to determine primary tuberculous infection was studied in 277 children and adolescents. The threshold diagnostic IFN-gamma induction level determined in the test tube containing a mixture of the antigens ESAT-6 and CFP-10 was ascertained. Postvaccine allergy was detectable if there was IFN-gamma induction in the test tube containing tuberculin and if there was no diagnostic IFN-gamma level in that containing the antigens ESAT-6 and CFP-10. The diagnostic sensitivity of detection of primary tuberculous infection was 97.6% with 94.4% specificity, which enabled this condition to be differentiated from postvaccine allergy. The level of antigen-induced IFN-gamma may be lower in relatively disseminated forms of pulmonary tuberculosis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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