| Autor: |
Qiang, Wei, Man-Ping, Wu, Pei-Fang, Chen, Mei-Zhen, Mei, Xin-Jian, Gao |
| Rok vydání: |
1996 |
| Zdroj: |
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica. 28(6) |
| ISSN: |
0582-9879 |
| Popis: |
The recombined (3)H-CE-HDL(2)(rHDL(2)) keeps the biological activities of the native HDL(2). After rat hepatic sinusoidal cells were incubated with rHDL(2) at 37 degrees for 3 h (normal group), the cell-endocytic cpm was 995-/+147(mean-/+s, n=2). After the cells were further incubated for 2 h, the cell-release TCA-precipitable cpm and the TCA-supernatant cpm were 78-/+32 and 12-/+9 respectively. These values were 339-/+62, 19-/+11 and 9-/+5 respectively in the acetylimidazole-modified group, and 542-/+78, 34-/+14 and 9-/+8 respectively in the heparin-pretreated group. Our results suggested that: (1) Rat hepatic sinusoidal cells internalize HDL(2) and take up HDL(2)-CE by its HDL receptor, and HDL(3) was secreted out of the cells by retroendocytosis. (2) Hepatic lipase (HL) induces directly the selective uptake of HDL(2)-CE by the cells. (3) There is cooperation between the HDL receptor and HL in the selective uptake of HDL(2)-CE by the cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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