Degradation of
| Autor: | Tien-Chiu, Wu, Yong-Han, Hong, Yung-Hsiang, Tsai, Shu-Ling, Hsieh, Ren-Han, Huang, Chia-Hung, Kuo, Chun-Yung, Huang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Membrane Potential
Mitochondrial Lung Neoplasms Cell Survival TOR Serine-Threonine Kinases Sargassum apoptosis Cytochromes c hydrogen peroxide Ascorbic Acid brown algae Article Sargassum crassifolium human lung carcinoma A-549 cells Proto-Oncogene Proteins c-bcl-2 fucoidan Polysaccharides anti-lung cancer Caspases Cell Line Tumor Humans Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Marine Drugs |
| ISSN: | 1660-3397 |
| Popis: | Fucoidans possess multiple biological functions including anti-cancer activity. Moreover, low-molecular-weight fucoidans are reported to possess more bioactivities than native fucoidans. In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced. The extrusion pretreatment can increase the extraction yield of fucoidan by approximately 4.2-fold as compared to the non-extruded sample. Among SC, SCA, SCH, and SCAH, the chemical compositions varied but structural features were similar. SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells. In general, SCA was found to exhibit high cytotoxicity to A-549 cells and a strong ability to suppress Bcl-2 expression. SCA also showed high efficacy to induce cytochrome c release, activate caspase-9 and -3, and promote late apoptosis of A-549 cells. Therefore, our data suggest that SCA could have an adjuvant therapeutic potential in the treatment of lung cancer. Additionally, we explored that the Akt/mammalian target of rapamycin (mTOR) signaling pathway is involved in SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells. |
| Databáze: | OpenAIRE |
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