Antibody to the nonapeptide Glu-Glu-Glu-Glu-Tyr-Met-Pro-Met-Glu is specific for polyoma middle T antigen and inhibits in vitro kinase activity

Autor:	B, Schaffhausen, T L, Benjamin, L, Pike, J, Casnellie, E, Krebs
Rok vydání:	1982
Předmět:	Mice Antigens Polyomavirus Transforming Phosphotransferases Animals Chymotrypsin Antigen-Antibody Complex Kidney Polyomavirus Antigens T-Independent Oligopeptides Antibodies Cells Cultured Peptide Fragments
Zdroj:	The Journal of biological chemistry. 257(21)
ISSN:	0021-9258
Popis:	Middle T antigen of polyoma virus has an associated tyrosine kinase activity which phosphorylates tyrosine residue 315 on middle T in immunoprecipitates. A peptide representing the sequence of middle T from residue 311 to 319 has been synthesized. This peptide acts as a weak inhibitor of the kinase reaction. An antiserum has been raised against this peptide after conjugation to bovine serum albumin. The antibody is middle T-specific. Middle T antigen precipitated by this serum is largely inactive in the kinase reaction. Dissociation of the immune complex with peptide releases middle T in a kinase-active form.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::b82b04ef53cc0b83257cedf1a0d19ea7 https://pubmed.ncbi.nlm.nih.gov/6290466 Zobrazit plný text záznamu