Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya

Autor:	Frank L, Basiye, Margaret, Mbuchi, Charles, Magiri, George, Kirigi, Stijn, Deborggraeve, Gerard J, Schoone, Alfarazdeg A, Saad, Sayda, El-Safi, Enock, Matovu, Monique K, Wasunna
Rok vydání:	2010
Předmět:	Endemic Diseases Animals Humans Leishmaniasis Visceral Reproducibility of Results DNA Protozoan Kenya Polymerase Chain Reaction Sensitivity and Specificity RNA Protozoan Self-Sustained Sequence Replication Leishmania donovani
Zdroj:	Tropical medicineinternational health : TMIH. 15(7)
ISSN:	1365-3156
Popis:	To estimate the sensitivity and specificity of the OligoC-TesT and nucleic acid sequence-based amplification coupled to oligochromatography (NASBA-OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya.Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays.The Leishmania OligoC-TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90-98.8%) and a specificity of 88.8% (95% CI: 81-93.6%), while the sensitivity and specificity of the NASBA-OC were 79.8% (95% CI: 67-87%) and 100% (95% CI: 96.3-100%), respectively.Our findings indicate high sensitivity of the Leishmania OligoC-TesT on blood while the NASBA-OC is a better marker for active disease.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::b809131de091b6333f7bde5d6cf3013f https://pubmed.ncbi.nlm.nih.gov/20487428 Zobrazit plný text záznamu Plný text