[Estimation of human and animal immunological memory by testing the trogocytosis of virus-specific T lymphocytes]
| Autor: | A N, Naĭkhin, D A, Koren'kov, T V, Chirkova, G D, Petukhova, S A, Donina, L G, Rudenko |
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| Rok vydání: | 2011 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Adolescent Lymphoid Tissue Cell Membrane Vaccination Antigen-Presenting Cells Respiratory Mucosa CD8-Positive T-Lymphocytes Antibodies Viral Flow Cytometry Vaccines Attenuated Mice Young Adult Hyaluronan Receptors Influenza A Virus H1N1 Subtype Influenza Vaccines Antibody Formation Influenza Human Models Animal Mice Inbred CBA Animals Humans Female Immunologic Memory |
| Zdroj: | Voprosy virusologii. 56(5) |
| ISSN: | 0507-4088 |
| Popis: | This study is the first attempt to evaluate the immunogenicity of Russian live attenuated influenza reassortant influenza vaccine (LAIV), by using a modified T-cell recognition of antigen presenting cells by protein capture (TRAP) method. Single vaccination of 18-20-year-old volunteers with LAIV causes an increase in the peripheral blood levels of virus-specific memory CD4+ T lymphocytes. Some (40-60%) LAIV-vaccination volunteers respond to immunization by showing a significant elevation in the peripheral blood level of memory CD4+ T cells without a systemic humoral immune response recorded in the passive hemagglutination test. Vaccination of mice with live attenuated A (H1N1) influenza reassortant virus stimulates the production of memory CD8+CD44hi T lymphocytes in the nasal-associated lymphoid tissue, the entry of infection, so does influenza infection. Vaccination with inactivated A (H1N1) influenza virus practically fails to induce these cells. A (H1N1) influenza virus-specific CD8+CD44hi T lymphocytes remain within at least 2 months (observation time). The authors' modified TRAP may be used to evaluate virus-specific immunological T-cell memory after vaccination. |
| Databáze: | OpenAIRE |
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