| Autor: |
G A, Callaghan, C, Riordan, W S, Gilmore, I A, McIntyre, J M, Allen, B M, Hannigan |
| Rok vydání: |
1996 |
| Předmět: |
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| Zdroj: |
Lasers in surgery and medicine. 19(2) |
| ISSN: |
0196-8092 |
| Popis: |
The purpose of this study was to evaluate the possible role of reactive oxygen species (ROS) in mediating previously recorded alterations in DNA synthesis, inducible by low-intensity laser irradiation (LILI), in the haemopoietic cell line U937.The ability of LILI (660 nm, 12 mW, 5 kHz) to induce ROS from U937 cells was assessed spectrophotometrically at energy densities (E.D.) from 1.0 to 11.5 J/cm2. In order to assess whether laser-induced ROS could alter cellular proliferation DNA synthesis was measured post-irradiation, by the incorporation of tritiated thymidine (3H-TdR) into the cells in both the presence and absence of the antioxidant catalase (CAT).Detectable ROS were produced post-irradiation only from the differentiated form of the cell line. Analysis by Student's t-test for unrelated groups showed a significant difference, at E.D.s 2.9 and 8.6 J/cm2, in the extent of DNA synthesis occurring in cells irradiated in the presence of CAT or in its absence.These findings demonstrate that laser-inducible ROS can mediate laser's effects on this cell line. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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