Negative selection of peripheral blood stem cells to support a tandem autologous transplantation programme in multiple myeloma

Autor: Anna Maria, Barbui, Monica, Galli, Gianpietro, Dotti, Nadia, Belli, Gianmaria, Borleri, Giovanna, Gritti, Piermario, Bellavita, Piera, Viero, Benedetto, Comotti, Tiziano, Barbui, Alessandro, Rambaldi
Rok vydání: 2002
Předmět:
Zdroj: British journal of haematology. 116(1)
ISSN: 0007-1048
Popis: We recently described a two-step negative selection procedure whereby peripheral blood stem cells (PBSCs) were efficiently purged of contaminating neoplastic cells by a combination of monoclonal antibodies. Here, we report 60 newly diagnosed multiple myeloma (MM) patients treated with a double transplant programme and randomized to receive either unmanipulated or in vitro purged PBSCs. We demonstrated that this technique is feasible and safe without significant loss of either CD34+ or CD3+ cells. Haematological engraftment and immunological reconstitution were rapid without treatment-related mortality. Using polymerase chain reaction (PCR), we compared the level of minimal residual disease (MRD) in PBSC before and after in vitro purging and in vivo after transplant. A median of one tumour cell per 10(2) normal cells (range 10(1)-10(5)) was seen in the unmanipulated aphereses with a 3-4 log reduction after manipulation in vitro. However, despite this tumour debulking, all patients remained PCR positive in vivo. At 3 years, the estimated event-free survival was 40% in the control arm and 72% in the experimental arm (P = 0.05), whereas the estimated overall survival was 83% in both arms. This suggests that autologous transplantation using efficiently purged PBSCs can be performed safely, but confirms the need for innovative protocols for MRD eradication in vivo.
Databáze: OpenAIRE
Externí odkaz: