| Autor: |
D, Salariato, L A, Diorio, N, Mouso, F, Forchiassin |
| Rok vydání: |
2009 |
| Předmět: |
|
| Zdroj: |
Revista Argentina de microbiologia. 42(1) |
| ISSN: |
0325-7541 |
| Popis: |
Polygalacturonase (PG) production by Fomes sclerodermeus using solid-state fermentation (SSF) was carried out. Maximal PG activity (26 U/gdw) was obtained between days 11 and 13 at the end of exponential growth. PG activity in the crude extract was more stable at pH 5-6 and 30 degrees C and had optimum activity at pH 5 and 50 degrees C. Optimal conditions for PG extraction were: one time extraction with Na2SO4 as solvent with 10 min. of agitation. In a scale-up system, PG activity per gram of dry substrate decreased about 60% compared with the activity obtained in an Erlenmeyer flask; however, high total PG activity was obtained. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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