| Autor: |
H, Noorchashm, Y K, Lieu, S Y, Rostami, H K, Song, S A, Greeley, S, Bazel, C F, Barker, A, Naji |
| Rok vydání: |
1999 |
| Předmět: |
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| Zdroj: |
Transplantation. 67(9) |
| ISSN: |
0041-1337 |
| Popis: |
Direct measurement of the precursor frequency of alloreactive CD4+ T cells has been impossible due to the lack of a specific means of determining the absolute number of daughter cells generated with each division in a repertoire of stimulated T cells.Responder lymphocytes were fluorescently labeled and adoptively transferred into irradiated allogeneic stimulator mice or incubated in vitro with irradiated stimulator splenocytes. After a 65- to 70-hr stimulation period, responder cells were analyzed by flow cytometry.The precursor frequency of dividing CD4+ T cells was determined both in vivo and in vitro. The observed number of alloreactive daughter cells generated with each round of division was used to calculate the frequency of alloantigen-specific CD4+ T cells.A novel method for the direct calculation of the frequency of alloreactive CD4+ T cells is described. This technique allows the determination of changes in the frequency of alloreactive T cells that might underlie tolerance to alloantigens. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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