Characterization of
| Autor: | Ruben, Brandão, Rute, Marcelino, Fátima, Gonçalves, Isabel, Diogo, Ana, Carvalho, Joaquim, Cabanas, Inês, Costa, Pedro, Brogueira, Fernando, Ventura, Ana, Miranda, Kamal, Mansinho, Perpétua, Gomes |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male hepatitis C virus Genotype Hepacivirus resistance-associated substitutions Viral Nonstructural Proteins NS5A Antiviral Agents NS5B Article Cohort Studies Drug Resistance Viral Prevalence Humans Treatment Failure direct-acting antivirals Aged Aged 80 and over Fluorenes Portugal virus diseases Middle Aged Hepatitis C Amino Acid Substitution RNA Viral Benzimidazoles Female Sofosbuvir Uridine Monophosphate |
| Zdroj: | Viruses |
| ISSN: | 1999-4915 |
| Popis: | This study is focused on the prevalent NS5 coding region resistance-associated substitutions (RASs) in DAA-naive genotype (GT)1 HCV-infected patients and their potential impact on success rates. Plasma RNA from 81 GT1 HCV-infected patients was extracted prior to an in-house nested RT-PCR of the NS5 coding region, which is followed by Sanger population sequencing. NS5A RASs were present in 28.4% (23/81) of all GT1-infected patients with 9.9% (8/81) having the Y93C/H mutation. NS5B RASs showed a prevalence of 14.8% (12/81) and were only detected in GT1b. Overall 38.3% (31/81) of all GT1 HCV-infected patients presented baseline RASs. The obtained data supports the usefulness of resistance testing prior to treatment since a statistically significant association was found between treatment failure and the baseline presence of specific NS5 RASs known as Y93C/H (p = 0.04). |
| Databáze: | OpenAIRE |
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