The resolution of DNA fragments in capillary electrophoresis in replaceable agarose gels

Autor:	A, Palm, S, Hjertén
Rok vydání:	1996
Předmět:	Electrophoresis Agar Gel Oligodeoxyribonucleotides Restriction Mapping Electrophoresis Capillary Reproducibility of Results Indicators and Reagents DNA Buffers Hydrogen-Ion Concentration Sensitivity and Specificity Edetic Acid
Zdroj:	Journal of capillary electrophoresis. 3(3)
ISSN:	1079-5383
Popis:	The resolution of DNA restriction fragments in CE in replaceable agarose gels was investigated as a function of the concentration and pH of Tris-acetate ethylenediaminetetraacetic acid (EDTA) buffer both at constant migration time and at constant field strength. The resolution increased for all fragments when the buffer concentration was increased from 40 to 200 mM and the pH was lowered from 8.5 to 7.5. The resolution was lower in a Tris-borate-EDTA buffer in this pH range and lowest at pH 7.5.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::b59e9b9d0a723bc4ae4bad196451b3d0 https://pubmed.ncbi.nlm.nih.gov/9384750 Zobrazit plný text záznamu