Synthetic antigens as immunogens: Part IV. Specificity of antisera developed to Gal beta 1-3(GlcNAc beta 1-6)GalNAc and GlcNAc beta 1-6GalNAc

Autor:	K L, Matta, C F, Piskorz, S A, Abbas, K R, Diakun
Rok vydání:	1991
Předmět:	Vaccines Synthetic Carbohydrate Sequence Molecular Structure Antibody Specificity Molecular Sequence Data Serum Albumin Bovine Disaccharides Trisaccharides
Zdroj:	Immunological investigations. 20(5-6)
ISSN:	0882-0139
Popis:	Antisera to the chemically synthesized trisaccharide, Gal beta 1-3(GlcNAc beta 1-6)GalNAc, and disaccharide, GlcNAc beta 1-6GalNAc, were developed using the diazophenyl bovine serum albumin derivatives. The binding specificity of the antisera were analyzed by enzyme immunoassays with structurally related, chemically synthesized oligosaccharides. The anti-trisaccharide antibody showed no reactivity to T antigenic structures which bear the Gal beta 1-3GalNAc moiety. The immunodominant area of the Gal beta 1-3(GlcNAc beta 1-6)GalNAc was contained in the GlcNAc beta 1-6GalNAc region of the molecule. The reactivity pattern of the anti-trisaccharide antibody, although directed to the disaccharide, did not exactly duplicate the reactivity pattern of the anti-disaccharide.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::b5531c3d929ffe4f7a6a79191e268323 https://pubmed.ncbi.nlm.nih.gov/1791040 Zobrazit plný text záznamu