| Autor: |
Alexander F, Makarchikov, Tatsiana G, Kudyrka, Tatyana A, Luchko, Aliaksei V, Yantsevich, Iryna M, Rusina, Alena A, Makar, Iryna K, Kolas, Sergey A, Usanov |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Biochimica et biophysica acta. General subjects. 1866(4) |
| ISSN: |
1872-8006 |
| Popis: |
Adenosine thiamine triphosphate (AThTP) is a nucleotide discovered in bacteria and some other living organisms more than a decade ago. No biochemical function for AThTP has been established yet, however, experimental data available indicate its possible involvement in metabolic regulation or cell signaling. Metabolism of AThTP in mammals, as well as the feasibility of its pharmacological application, is essentially unstudied.Preparative low-pressure chromatography was employed to purify chemically synthesized AThTP with its further analysis by mass spectrometry, HPLC, UV and fluorescence spectroscopy. Enzyme activity assays along with HPLC were used to examine the effects of AThTP and thiamine on vitamin BAn improved procedure for AThTP synthesis and purification is elaborated. Solution stability, optical spectral properties and the molar absorption coefficient for AThTP were determined. The levels of thiamine compounds were found to be increased in the liver of diabetic rats. Neither AThTP nor thiamine treatment affected hepatic vitamin BContrast to the widespread view about thiamine deficiency in diabetes, our results clearly shows an adaptive increase in the level of B |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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