Regulation of insulin-like growth factor binding proteins in young growing animals by alteration of energy status
| Autor: | M J, Dauncey, B T, Rudd, D A, White, R A, Shakespear |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Aging
Swine Blotting Western Growth Insulin-Like Growth Factor Binding Protein 1 Insulin-Like Growth Factor Binding Proteins Molecular Weight Eating Insulin-Like Growth Factor Binding Protein 2 Insulin-Like Growth Factor Binding Protein 4 Animals Autoradiography Electrophoresis Polyacrylamide Gel Insulin-Like Growth Factor I Carrier Proteins Energy Metabolism Body Temperature Regulation Densitometry |
| Zdroj: | Growth regulation. 3(3) |
| ISSN: | 0956-523X |
| Popis: | The regulation of plasma insulin-like growth factor binding proteins (IGFBPs) by energy status has been assessed in 2-month-old pigs. Energy balance was modified by altering thermoregulatory demand and energy intake, with litter-mates being kept for several weeks at either 35 or 10 degrees C on a high (H) or low (L) level of food intake (where H = 2L); plasma samples were taken 20-24 h after the last meal. The two major forms of circulating IGFBP, as estimated by Western blot analysis, were identified putatively as IGFBP-2 and IGFBP-3 (relative molecular weights of 34 and 40-45 kDa respectively). There were significant differences in IGFBP profiles between the four treatment groups of 35H, 35L, 10H and 10L: the 40-45 kDa IGFBP (putative IGFBP-3) was elevated both in the warm and on a high food intake (P0.001), and there was a marked reciprocal relation between the 40-45 and 34 kDa IGFBPs. The relative concentration of the 34 kDa IGFBP (putative IGFBP-2) was greatest in the 10L and least in the 35H group. It is concluded that long-term alterations in energy balance, induced by changes in either intake or thermoregulatory demand, can significantly affect the plasma profile of IGFBPs during the first two months of life. |
| Databáze: | OpenAIRE |
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