| Autor: |
Y, Yamashita, S, Sendo, T, Hosokawa, G, Tuszynski, T, Kurohiji, R, Beppu, T, Shinohara, T, Kinugasa, T, Shirakusa |
| Rok vydání: |
1998 |
| Předmět: |
|
| Zdroj: |
International journal of oncology. 13(2) |
| ISSN: |
1019-6439 |
| Popis: |
The effects of thrombospondin (TSP) on the proliferation of two different human carcinoma cell lines (KIM-1 and CW-2) were investigated. The characterization of these two carcinoma cells by immunohistochemistry using anti-TSP antibody and anti-TSP-receptor antibody showed that the KIM-1 had TSP-receptors and TSP, while the CW-2 had only TSP-receptors. The addition of exogenous TSP (10 or 20 microg/ml) to culture medium stimulated the cell proliferation of CW-2 but not that of KIM-1. The cell count for CW-2 was increased dosage-dependently from 10.3 0.6x104/ml at zero TSP concentration to 12.9 0.6x104/ml at 10 microg/ml TSP concentration and to 14.7 0. 4x104/ml at 20 microg/ml TSP (each p0.0001). In conclusion, though TSP promoted the proliferation of non-TSP-producing cells, it did not promote proliferation of TSP-producing cells. Therefore, it is predicted that TSP was already at saturated activity concentration in the TSP-producing cell line (KIM-1). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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