| Autor: |
S, Bompensieri, R, Gonzalez, R, Kok, M V, Miranda, I, Nutgeren-Roodzant, K J, Hellingwerf, O, Cascone, B C, Nudel |
| Rok vydání: |
1996 |
| Předmět: |
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| Zdroj: |
Biotechnology and applied biochemistry. 23(1) |
| ISSN: |
0885-4513 |
| Popis: |
The performance of hydrophobic-interaction chromatography (HIC) for the purification of Acinetobacter calcoaceticus AAC323-1 lipase was compared with that of various aqueous two-phase systems. While a 42% lipase yield with a purification factor of 140 could be recovered by HIC, higher yields were achieved by using aqueous two-phase systems, either those formed by poly(ethylene glycol) and dextran or those based upon the use of a detergent. Triton X-114-based aqueous two-phase partition showed the best performance, with a yield of 81% and a purification factor of 68. Further detergent removal was easily achieved with an adsorbent, with no significant decrease in yields. Owing to its simplicity, the method should be easy to scale-up. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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