Complementation of a pgk deletion mutation in Saccharomyces cerevisiae with expression of the phosphoglycerate-kinase gene from the hyperthermophilic Archaeon Sulfolobus solfataricus

Autor:	P W, Piper, C, Emson, C E, Jones, D A, Cowan, T M, Fleming, J A, Littlechild
Rok vydání:	1996
Předmět:	Phosphoglycerate Kinase Transformation Genetic Base Sequence Genes Bacterial Enzyme Stability Genes Fungal Genetic Complementation Test Molecular Sequence Data Gene Expression Saccharomyces cerevisiae Gene Deletion DNA Primers Sulfolobus
Zdroj:	Current genetics. 29(6)
ISSN:	0172-8083
Popis:	The gene encoding phosphoglycerate kinase (PGK) from the Archaeon Sulfolobus solfataricus, an organism growing optimally at 87 degrees C, was inserted into a yeast expression vector under the control of the galactose-inducible GAL1 yeast promoter. This vector was then transformed into a pgk::TRP1 yeast mutant, a strain inhibited for growth on galactose or glucose due to its lack of PGK enzyme. Slow-growing transformants were obtained on galactose plates at 37 degrees C, but not 28 degrees C. These transformants contained low levels of transcripts of the heterologous gene and low amounts of thermostable PGK activity. Weak expression of the hyperthermophile gene in yeast, a mesophile, therefore enabled complementation of the yeast pgk defect at 37 degrees C but not at 28 degrees C.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::b3fb8fc0b72fec3280e34ae46ecab48a https://pubmed.ncbi.nlm.nih.gov/8662201 Zobrazit plný text záznamu Full text from SpringerLink